Faculté de médecine 
et des sciences de la santéhttp://www.usherbrooke.ca/medecine

Groupe de recherche du Professeur Richard Leduc    -    Département de Pharmacologie    -    Richard.Leduc@USherbrooke.ca

Publications

Identification of transmembrane domain 3, 4 & 5 residues that contribute to the formation of the ligand-binding pocket of the urotensin-II receptor.


Sainsily X, Cabana J, Boulais PE, Holleran BJ, Escher E, Lavigne P, Leduc R.

Biochem Pharmacol. 2013 Sep 28. doi:pii: S0006-2952(13)00620-5. 10.1016/j.bcp.2013.09.015. [Epub ahead of print]

PMID: 24084430 [PubMed - as supplied by publisher]

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Matriptase proteolytically activates influenza virus and promotes multicycle replication in the human airway epithelium.


Beaulieu A, Gravel É, Cloutier A, Marois I, Colombo É, Désilets A, Verreault C, Leduc R, Marsault É, Richter MV.

J Virol. 2013 Apr;87(8):4237-51. doi: 10.1128/JVI.03005-12. Epub 2013 Jan 30.

PMID: 23365447 [PubMed - indexed for MEDLINE] Free PMC Article

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Analysis by substituted cysteine scanning mutagenesis of the fourth transmembrane domain of the CXCR4 receptor in its inactive and active state.


Boulais PE, Escher E, Leduc R.

Biochem Pharmacol. 2013 Feb 15;85(4):541-50. doi: 10.1016/j.bcp.2012.11.022. Epub 2012 Dec 5.

PMID: 23219524 [PubMed - indexed for MEDLINE]

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Critical hydrogen bond formation for activation of the angiotensin II type 1 receptor.


Cabana J, Holleran B, Beaulieu MÈ, Leduc R, Escher E, Guillemette G, Lavigne P.

J Biol Chem. 2013 Jan 25;288(4):2593-604. doi: 10.1074/jbc.M112.395939. Epub 2012 Dec 7.

PMID: 23223579 [PubMed - indexed for MEDLINE]

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Matriptase protects against experimental colitis and promotes intestinal barrier recovery.


Netzel-Arnett S, Buzza MS, Shea-Donohue T, Désilets A, Leduc R, Fasano A, Bugge TH, Antalis TM.

Inflamm Bowel Dis. 2012 Jul;18(7):1303-14. doi: 10.1002/ibd.21930. Epub 2011 Nov 13.

PMID: 22081509 [PubMed - indexed for MEDLINE] Free PMC Article

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Elucidation of the structure-activity relationships of apelin: influence of unnatural amino acids on binding, signaling, and plasma stability.


Murza A, Parent A, Besserer-Offroy E, Tremblay H, Karadereye F, Beaudet N, Leduc R, Sarret P, Marsault É.

ChemMedChem. 2012 Feb 6;7(2):318-25. doi: 10.1002/cmdc.201100492. Epub 2011 Dec 13.

PMID: 22170700 [PubMed - indexed for MEDLINE]

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Essential role of endocytosis of the type II transmembrane serine protease TMPRSS6 in regulating its functionality.


Béliveau F, Brulé C, Désilets A, Zimmerman B, Laporte SA, Lavoie CL, Leduc R.

J Biol Chem. 2011 Aug 19;286(33):29035-43. doi: 10.1074/jbc.M111.223461. Epub 2011 Jul 1.

PMID: 21724843 [PubMed - indexed for MEDLINE] Free PMC Article

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Influence of Ca2+ and pH on the folding of the prourotensin II precursor.


Bilodeau J, Désilets A, McDuff FO, St-Pierre C, Barbar E, Leduc R, Lavigne P.

FEBS Lett. 2011 Jun 23;585(12):1910-4. doi: 10.1016/j.febslet.2011.04.075. Epub 2011 May 19.

PMID: 21605557 [PubMed - indexed for MEDLINE]

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Temperature dependent photolabeling of the human angiotensin II type 1 receptor reveals insights into its conformational landscape and its activation mechanism.


Arsenault J, Cabana J, Fillion D, Leduc R, Guillemette G, Lavigne P, Escher E.

Biochem Pharmacol. 2010 Oct 1;80(7):990-9. doi: 10.1016/j.bcp.2010.06.004. Epub 2010 Jun 19.

PMID: 20599798 [PubMed - indexed for MEDLINE]

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A single-nucleotide polymorphism of alanine to threonine at position 163 of the human angiotensin II type 1 receptor impairs Losartan affinity.


Arsenault J, Lehoux J, Lanthier L, Cabana J, Guillemette G, Lavigne P, Leduc R, Escher E.

Pharmacogenet Genomics. 2010 Jun;20(6):377-88. doi: 10.1097/FPC.0b013e32833a6d4a.

PMID: 20436376 [PubMed - indexed for MEDLINE]

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The amino-terminus of angiotensin II contacts several ectodomains of the angiotensin II receptor AT1.


Fillion D, Lemieux G, Basambombo LL, Lavigne P, Guillemette G, Leduc R, Escher E.

J Med Chem. 2010 Mar 11;53(5):2063-75. doi: 10.1021/jm9015747.

PMID: 20146480 [PubMed - indexed for MEDLINE]

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Analysis of transmembrane domains 1 and 4 of the human angiotensin II AT1 receptor by cysteine-scanning mutagenesis.


Yan L, Holleran BJ, Lavigne P, Escher E, Guillemette G, Leduc R.

J Biol Chem. 2010 Jan 22;285(4):2284-93. doi: 10.1074/jbc.M109.077180. Epub 2009 Nov 23.

PMID: 19940150 [PubMed - indexed for MEDLINE] Free PMC Article

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Photolabeling identifies transmembrane domain 4 of CXCR4 as a T140 binding site.


Boulais PE, Dulude D, Cabana J, Heveker N, Escher E, Lavigne P, Leduc R.

Biochem Pharmacol. 2009 Dec 1;78(11):1382-90. doi: 10.1016/j.bcp.2009.07.007. Epub 2009 Jul 22.

PMID: 19631193 [PubMed - indexed for MEDLINE]

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The fifth transmembrane domain of angiotensin II Type 1 receptor participates in the formation of the ligand-binding pocket and undergoes a counterclockwise rotation upon receptor activation.


Domazet I, Martin SS, Holleran BJ, Morin ME, Lacasse P, Lavigne P, Escher E, Leduc R, Guillemette G.

J Biol Chem. 2009 Nov 13;284(46):31953-61. doi: 10.1074/jbc.M109.051839. Epub 2009 Sep 22.

PMID: 19773549 [PubMed - indexed for MEDLINE] Free PMC Article

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Activation induces structural changes in the liganded angiotensin II type 1 receptor.


Clément M, Cabana J, Holleran BJ, Leduc R, Guillemette G, Lavigne P, Escher E.

J Biol Chem. 2009 Sep 25;284(39):26603-12. doi: 10.1074/jbc.M109.012922. Epub 2009 Jul 27.

PMID: 19635801 [PubMed - indexed for MEDLINE] Free PMC Article

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Characterization of proADAMTS5 processing by proprotein convertases.


Longpré JM, McCulloch DR, Koo BH, Alexander JP, Apte SS, Leduc R.

Int J Biochem Cell Biol. 2009 May;41(5):1116-26. doi: 10.1016/j.biocel.2008.10.008. Epub 2008 Nov 1.

PMID: 18992360 [PubMed - indexed for MEDLINE]

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The second transmembrane domain of the human type 1 angiotensin II receptor participates in the formation of the ligand binding pocket and undergoes integral pivoting movement during the process of receptor activation.


Domazet I, Holleran BJ, Martin SS, Lavigne P, Leduc R, Escher E, Guillemette G.

J Biol Chem. 2009 May 1;284(18):11922-9. doi: 10.1074/jbc.M808113200. Epub 2009 Mar 9.

PMID: 19276075 [PubMed - indexed for MEDLINE] Free PMC Article

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Identification of transmembrane domain 6 & 7 residues that contribute to the binding pocket of the urotensin II receptor.


Holleran BJ, Domazet I, Beaulieu ME, Yan LP, Guillemette G, Lavigne P, Escher E, Leduc R.

Biochem Pharmacol. 2009 Apr 15;77(8):1374-82. doi: 10.1016/j.bcp.2009.01.013. Epub 2009 Jan 29.

PMID: 19426676 [PubMed - indexed for MEDLINE]

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Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides.


Béliveau F, Désilets A, Leduc R.

FEBS J. 2009 Apr;276(8):2213-26. doi: 10.1111/j.1742-4658.2009.06950.x. Epub 2009 Mar 3.

PMID: 19302215 [PubMed - indexed for MEDLINE]

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Structural changes in the binding pocket of the liganded angiotensin II receptor AT1 during activation.


Clément M, Guillemette G, Leduc R, Escher E.

Adv Exp Med Biol. 2009;611:341-2. No abstract available.

PMID: 19400219 [PubMed - indexed for MEDLINE]

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Lysosomotropic drugs inhibit maturation of transforming growth factor-beta.


Basque J, Martel M, Leduc R, Cantin AM.

Can J Physiol Pharmacol. 2008 Sep;86(9):606-12. doi: 10.1139/y08-063.

PMID: 18758509 [PubMed - indexed for MEDLINE]

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Mutational analysis of the conserved Asp2.50 and ERY motif reveals signaling bias of the urotensin II receptor.


Proulx CD, Holleran BJ, Boucard AA, Escher E, Guillemette G, Leduc R.

Mol Pharmacol. 2008 Sep;74(3):552-61. doi: 10.1124/mol.108.045054. Epub 2008 May 28.

PMID: 18509066 [PubMed - indexed for MEDLINE] Free Article

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Biological properties and functional determinants of the urotensin II receptor.


Proulx CD, Holleran BJ, Lavigne P, Escher E, Guillemette G, Leduc R.

Peptides. 2008 May;29(5):691-9. Epub 2007 Nov 17. Review.

PMID: 18155322 [PubMed - indexed for MEDLINE]

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Mutation G827R in matriptase causing autosomal recessive ichthyosis with hypotrichosis yields an inactive protease.


Désilets A, Béliveau F, Vandal G, McDuff FO, Lavigne P, Leduc R.

J Biol Chem. 2008 Apr 18;283(16):10535-42. doi: 10.1074/jbc.M707012200. Epub 2008 Feb 8.

PMID: 18263585 [PubMed - indexed for MEDLINE] Free PMC Article

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Activation of the angiotensin II type 1 receptor leads to movement of the sixth transmembrane domain: analysis by the substituted cysteine accessibility method.


Martin SS, Holleran BJ, Escher E, Guillemette G, Leduc R.

Mol Pharmacol. 2007 Jul;72(1):182-90. Epub 2007 Apr 19.

PMID: 17446269 [PubMed - indexed for MEDLINE] Free Article

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Inhibition of human matriptase by eglin c variants.


Désilets A, Longpré JM, Beaulieu ME, Leduc R.

FEBS Lett. 2006 Apr 17;580(9):2227-32. Epub 2006 Mar 20.

PMID: 16566926 [PubMed - indexed for MEDLINE]

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Role of N-glycan-dependent quality control in the cell-surface expression of the AT1 receptor.


Lanctôt PM, Leclerc PC, Escher E, Guillemette G, Leduc R.

Biochem Biophys Res Commun. 2006 Feb 10;340(2):395-402. Epub 2005 Dec 13.

PMID: 16364240 [PubMed - indexed for MEDLINE]

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The active and the inactive form of the hAT1 receptor have an identical ligand-binding environment: an MPA study on a constitutively active angiotensin II receptor mutant.


Clément M, Chamberland C, Pérodin J, Leduc R, Guillemette G, Escher E.

J Recept Signal Transduct Res. 2006;26(5-6):417-33.

PMID: 17118790 [PubMed - indexed for MEDLINE]

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Mass spectrometric peptide fingerprinting of proteins after Western blotting on polyvinylidene fluoride and enhanced chemiluminescence detection.


Methogo RM, Dufresne-Martin G, Leclerc P, Leduc R, Klarskov K.

J Proteome Res. 2005 Nov-Dec;4(6):2216-24.

PMID: 16335969 [PubMed - indexed for MEDLINE]

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Importance of N-glycosylation positioning for cell-surface expression, targeting, affinity and quality control of the human AT1 receptor.


Lanctot PM, Leclerc PC, Clément M, Auger-Messier M, Escher E, Leduc R, Guillemette G.

Biochem J. 2005 Aug 15;390(Pt 1):367-76.

PMID: 15869468 [PubMed - indexed for MEDLINE] Free PMC Article

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The constitutively active N111G-AT1 receptor for angiotensin II modifies the morphology and cytoskeletal organization of HEK-293 cells.


Auger-Messier M, Turgeon ES, Leduc R, Escher E, Guillemette G.

Exp Cell Res. 2005 Aug 1;308(1):188-95.

PMID: 15896777 [PubMed - indexed for MEDLINE]

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Determining the environment of the ligand binding pocket of the human angiotensin II type I (hAT1) receptor using the methionine proximity assay.


Clément M, Martin SS, Beaulieu ME, Chamberland C, Lavigne P, Leduc R, Guillemette G, Escher E.

J Biol Chem. 2005 Jul 22;280(29):27121-9. Epub 2005 May 12.

PMID: 15890659 [PubMed - indexed for MEDLINE] Free Article

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Stability of mutant serpin/furin complexes: dependence on pH and regulation at the deacylation step.


Dufour EK, Désilets A, Longpré JM, Leduc R.

Protein Sci. 2005 Feb;14(2):303-15.

PMID: 15659365 [PubMed - indexed for MEDLINE] Free PMC Article

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Involvement of a cytoplasmic-tail serine cluster in urotensin II receptor internalization.


Proulx CD, Simaan M, Escher E, Laporte SA, Guillemette G, Leduc R.

Biochem J. 2005 Jan 1;385(Pt 1):115-23.

PMID: 15458389 [PubMed - indexed for MEDLINE] Free PMC Article

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Analysis of the third transmembrane domain of the human type 1 angiotensin II receptor by cysteine scanning mutagenesis.


Martin SS, Boucard AA, Clément M, Escher E, Leduc R, Guillemette G.

J Biol Chem. 2004 Dec 3;279(49):51415-23. Epub 2004 Sep 27.

PMID: 15452107 [PubMed - indexed for MEDLINE] Free Article

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Down-regulation of inositol 1,4,5-trisphosphate receptor in cells stably expressing the constitutively active angiotensin II N111G-AT(1) receptor.


Auger-Messier M, Arguin G, Chaloux B, Leduc R, Escher E, Guillemette G.

Mol Endocrinol. 2004 Dec;18(12):2967-80. Epub 2004 Aug 26.

PMID: 15331757 [PubMed - indexed for MEDLINE] Free Article

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Identification of prodomain determinants involved in ADAMTS-1 biosynthesis.


Longpré JM, Leduc R.

J Biol Chem. 2004 Aug 6;279(32):33237-45. Epub 2004 Jun 7.

PMID: 15184385 [PubMed - indexed for MEDLINE] Free Article

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The constitutively active N111G-AT1 receptor for angiotensin II maintains a high affinity conformation despite being uncoupled from its cognate G protein Gq/11alpha.


Auger-Messier M, Clement M, Lanctot PM, Leclerc PC, Leduc R, Escher E, Guillemette G.

Endocrinology. 2003 Dec;144(12):5277-84. Epub 2003 Aug 13.

PMID: 12960024 [PubMed - indexed for MEDLINE] Free Article

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Constitutive activation of the angiotensin II type 1 receptor alters the spatial proximity of transmembrane 7 to the ligand-binding pocket.


Boucard AA, Roy M, Beaulieu ME, Lavigne P, Escher E, Guillemette G, Leduc R.

J Biol Chem. 2003 Sep 19;278(38):36628-36. Epub 2003 Jul 3.

PMID: 12842881 [PubMed - indexed for MEDLINE] Free Article

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Photolabelling the rat urotensin II/GPR14 receptor identifies a ligand-binding site in the fourth transmembrane domain.


Boucard AA, Sauvé SS, Guillemette G, Escher E, Leduc R.

Biochem J. 2003 Mar 15;370(Pt 3):829-38.

PMID: 12495432 [PubMed - indexed for MEDLINE] Free PMC Article

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Residues 293 and 294 are ligand contact points of the human angiotensin type 1 receptor.


Pérodin J, Deraët M, Auger-Messier M, Boucard AA, Rihakova L, Beaulieu ME, Lavigne P, Parent JL, Guillemette G, Leduc R, Escher E.

Biochemistry. 2002 Dec 3;41(48):14348-56.

PMID: 12450401 [PubMed - indexed for MEDLINE]

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Polyethylene glycol conjugation at Cys232 prolongs the half-life of alpha1 proteinase inhibitor.


Cantin AM, Woods DE, Cloutier D, Dufour EK, Leduc R.

Am J Respir Cell Mol Biol. 2002 Dec;27(6):659-65.

PMID: 12444025 [PubMed - indexed for MEDLINE]

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A polyaromatic caveolin-binding-like motif in the cytoplasmic tail of the type 1 receptor for angiotensin II plays an important role in receptor trafficking and signaling.


Leclerc PC, Auger-Messier M, Lanctot PM, Escher E, Leduc R, Guillemette G.

Endocrinology. 2002 Dec;143(12):4702-10.

PMID: 12446598 [PubMed - indexed for MEDLINE] Free Article

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Ectodomain shedding of furin: kinetics and role of the cysteine-rich region.


Denault J, Bissonnette L, Longpré J, Charest G, Lavigne P, Leduc R.

FEBS Lett. 2002 Sep 11;527(1-3):309-14.

PMID: 12220680 [PubMed - indexed for MEDLINE]

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Processing of proendothelin-1 by members of the subtilisin-like pro-protein convertase family.


Blais V, Fugère M, Denault JB, Klarskov K, Day R, Leduc R.

FEBS Lett. 2002 Jul 31;524(1-3):43-8.

PMID: 12135739 [PubMed - indexed for MEDLINE]

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Inhibitory potency and specificity of subtilase-like pro-protein convertase (SPC) prodomains.


Fugère M, Limperis PC, Beaulieu-Audy V, Gagnon F, Lavigne P, Klarskov K, Leduc R, Day R.

J Biol Chem. 2002 Mar 8;277(10):7648-56. Epub 2001 Nov 26.

PMID: 11723118 [PubMed - indexed for MEDLINE] Free Article

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